TCBES MS Thesis Defense via Zoom - Ryan Domingo - Event Details

This event has concluded and is no longer current.

This event is being held online. Password: 129079

TCBES MS Thesis Defense via Zoom - Ryan Domingo

Location: Online

TCBES Master's Thesis Defense via Zoom - Ryan Domingo
Wednesday, April 15, 2020, 12:30 PM
Location: Online Only

Title: Applying Meristem Tip Culture and Thermotherapy to Eliminate Viruses from Pineapple (Ananas comosus) and Taro (Colocasia esculenta)

Join Zoom Meeting: hawaii.zoom.us/j/977939411
Password: 129079

Abstract: Plant meristem tip culture and thermotherapy were evaluated for eliminating viruses from pineapple and taro. These crops are vegetatively propagated, so virus elimination methods are vital to rescuing infected lines. Pineapple mealybug wilt associated viruses (PMWaV) cause mealybug wilt of pineapple disease (MWP) in addition to a general loss of plant vigor. I evaluated meristem tip culture, thermotherapy or a combination of the two for their utility in eliminating PMWaV from pineapple tissue. I also compared bud explant sizes (1mm and 2mm), and bud excision locations from the mother plant (top, middle, and bottom thirds) for eliminating PMWaVs, as well as bud survival and plantlet growth rates. Plantlets from 1mm buds were free from PMWaV in 97% of plantlets tested >9 months after initiation versus 83% of the plantlets from 2mm explants. Excising buds from upper positions on the mother plant was more effective for virus exclusion than from lower positions. About 97% of the buds harvested from the top third of the plant yielded virus-free plantlets, compared to 85% from the middle, and 74% from the bottom. Overall, 399 buds were initiated and 193 (48%) survived. While 1mm buds provided more virus-free plantlets, 2mm buds (N=229) had a 16% better chance of survival. The best bud survival (67%) was observed in 2mm buds harvested from the top section. Growth measurements during thermotherapy showed that buds excised from top sections produced faster-growing plantlets (0.51mm/day) than those from middle sections (0.085mm/day). At 37°C, plantlets from top sections grew 0.26mm/d; at 35°C, 0.51mm/d; at 30°C, 0.62mm/d; and at room temperature 0.65mm/d. Taro vein chlorosis virus (TaVCV) was recently found in Hawaii, and can cause severe symptoms and crop loss. As with the pineapple experiments, I conducted meristem tip culture using different bud explant sizes (1mm and 2mm) and different bud locations along the mother plant (top, middle-bottom) to determine its efficacy in eliminating TaVCV and overall bud survival rates during the early stages of meristem tip culture. Thermotherapy treatment was also conducted, to measure the in vitro growth rates of taro (top) during temperature treatments (37°C, 35°C, 30°C, RT), for future virus elimination from taro. For taro, initiating 2mm bud explant sizes was as effective as initiating 1mm explants in eliminating TaVCV (P > 0.25). Both explant sizes were able to rid TaVCV from 97% of all plants maintained in vitro after testing at >1 year. No correlation between bud location and TaVCV presence was detected (P >1). However, a correlation between taro varieties (M. ulu, purple, and purple2) and TaVCV presence was observed (P < 0.046). Purple-2 had all (100%, N=13) buds tested negative while the Purple variety had (98.5%, N=67) free of TaVCV. Variety M. ulu had the lowest rate of clean plants (77.7%, N=11). Bud survival rates among 1mm and 2mm buds for the first month were nearly identical at 89% and 90%, however, buds harvested from the top location had an 8.5% higher survival rate. At 35°C, taro explants grew 0.58mm/day, while at 30°C plants grew 1.52mm/day. The implications for including these methods in virus elimination protocols are discussed.

For more information, contact: uhhtcbes@hawaii.edu 8089327573

Tags: ryan domingo tcbes research meristem virus taro pineapple Online Only

What's also happening?

Announcements

Student Activities Council (SAC) Open Positions - Apply Now!
SAC Applications are open. The Student Activities Council (SAC) provides programs, activities and services which serve the co-curricular cultural, social, recreational and educational interests of students of the University of Hawaiʻi at Hilo. ...
Student Activities Council (SAC) ID Validation Reminder
Aloha Vulcans, Hope you are all ready for this upcoming Fall semester. Best of luck to all students both returning and freshmen from all of SAC. ...
BOSP Positions Open!
If you enjoy writing, editing or leadership join BOSP! The Board of Student Publications has positions open in Hohonu, Kanilehua, Ke Kalahea and the Board! Applications are available on our website under Forms. ...
Menstrual Equity Survey
We want to hear from you! The Women's Center and UHHSA are collaborating on a menstrual equity initiative that could bring a free supply of period products on campus. Fill out out the menstrual equity survey using this link: ...
Diaper Bank Flyer
Do you want to donate diapers for our locals and keiki? Come by the new Hawai`i Diaper Bank drop off location with your diaper donation!
AANAPISI Scholarship
UH Hilo undergraduate students of Native Hawaiian, Pacific Islander, and Asian American backgrounds who are U.S. ...
APIA Scholarship
UH Hilo undergraduate students of Native Hawaiian, Pacific Islander, and Asian American backgrounds who are U.S. ...
2022-23 UH Common Scholarship Application Available Oct 1st
Starting October 1, 2021, students can complete the 2022-23 UH System Common Scholarship Application online at www.hawaii.edu/tuition/scholarships/how-to-apply/. ...
RISO Registration for 2021-2022 is now open
The 2021-2022 academic year UH Hilo Registered Independent Student Organization (RISO) registration period is now open. The final day to register is March 25, 2022, at 4:30 pm. ...
2022-23 FAFSA Available October 1st
Starting October 1, 2021, students can complete their 2022-23 Free Application for Federal Student Aid (FAFSA) online at Studentaid.gov/fafsa To receive priority consideration at UH Hilo, complete your FAFSA by February 1, 2022. ...
Cultivating Pilina: Empowering Connection to End DV
Aloha! Please join us every Friday for the month of October to engage in courageous conversations around Domestic Violence, as we work collectively to keep our communities safe. ...

Disclaimer: The University of Hawaii at Hilo is not responsible for the contents, links and/or materials presented in any web site listed above that is not of the “hilo.hawaii.edu” or “.uhh.hawaii.edu” domains. All comments, complaints and grievances should be filed with the author, host and/or owner of said site.