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Workshops

UH Hilo Home > Department Web Sites > EPSCoR Hilo Core Genetics Facility

Overview

The facility runs workshops and training sessions to introduce current molecular and biotechnological techniques to anyone in the community who is interested, including community college, high-school, and middle-school students and faculty statewide. Current workshops include DNA isolation, PCR Basics, Gel Electrophoresis, Microsatellites Basics, t-RFLP Basics, Real-time PCR Basics, Molecular Cloning, and DNA Sequencing. The lab now charges a fee for these training workshops.

Current Workshops Available

DNA Extraction

DNA moleculeThis workshop provides an introduction to DNA extraction from different organisms, including the theory behind the procedure and current techniques in use today. This lecture is followed by a lab practicum that includes hands-on experience in which participants will extract DNA, from the organism of their choice, using two currently available kits from Promega and Qiagen. The resulting DNA sample will be analyzed using gel electrophoresis and spectrophotometry. (Duration 3-4hrs)

PCR Basics

pcr cycleThis workshop provides an introduction to the polymerase chain reaction (PCR), including the theory behind the PCR, and the conditions and reagents needed for optimization of PCR. This lecture is followed by a lab practicum that includes hands-on experience in which participants will set up a PCR reaction and amplify a fragment of bacteriophage lambda DNA. The resulting PCR sample will be analyzed using gel electrophoresis and spectrophotometry. (Duration 3-4hrs)

Gel Electrophoresis Basics:

gel photoThis workshop provides an introduction to gel electrophoresis, including the theory behind electrophoresis and the reagents used in the procedure. This lecture is followed by a lab practicum that includes hands-on experience in which participants will have the opportunity to pour gels, load samples, and stain and photograph the gel. (Duration 3-4hrs)

Introduction to Molecular Cloning

plamid photoThis workshop provides an introduction to molecular cloning using the TOPO-TA Cloning Kit (Invitrogen), including the theory and overview of the procedure. The sessions will include both lecture and lab portions in which participants will be taken through the step-by-step procedure of cloning a DNA fragment and analyzing the results, including plasmid preps, restriction and PCR analysis. (Duration 3days, 1-4hrs/day)

DNA Sequencing

DNA sequence

This workshop provides an introduction to DNA sequencing, including the theory and overview of the procedure. The lecture is followed by a lab practicum that includes preparation of a DNA sequencing reaction, set-up of the PCR reaction, post-PCR clean-up reaction, and sequencing instrument set-up. After the sequencing run is complete, an overview of the raw data analysis and interpretation of results is performed. This is an advanced training session and basics training workshops are required prior to attending this workshop. (Duration 2 days, 2 hrs/day).

Real-Time PCR Basics

real time PCR

This workshop provides an introduction to fluorescent-based polymerase chain reaction (PCR) reagents to provide quantitative detection of target DNA fragments using real-time analysis, qualitative detection of targets using post-PCR analysis, and qualitative analysis of the PCR product using post-PCR Melt Curve analysis. The hands-on portion of this training session includes the preparation of a relative standard curve and comparative CT experiment.

Microsatellites Basics

DNA sequence of microsatellite repeat

This workshop provides an introduction to fragment analysis of DNA short tandem repeats (STR), also known as microsatellites. This lecture is followed by a lab practicumDNA microsatellites chromatogram that includes the amplification of microsatellite fragments using PCR techniques. The amplified fragments are visualized on an agarose gel and prepared for DNA fragment analysis. After the run the results are visualized and analyzed. (Duration 3 days, 1-2 hrs/day).

tRFLP Basics

Bacteria colonies in dirt sampleChromatogram of bacteria colonies isolated in tRFLP experiment

This workshop initiates to Terminal Restriction Fragment Length Polymorphism, a molecular genetics fingerprinting technique that is typically used to identify mixed-species of bacterial community present in a substrate. The 16 S rDNA gene, a well conserved gene present among microbial communities is PCR amplified, cleaned-up and digested overnight with restriction enzymes. The mixture of digested fragments is separated by automated electrophoresis, and fragment sizes are detected by fluorescent detection. Each peak observed from the graphic output corresponds to a specific microbial colony. This is an advanced training session, and basics training workshops are required prior to attending this workshop. (4 days, 21/2 hrs/day).

Make an Appointment

Contact lab manager Anne Veillet at 932-7571 or email aveillet@hawaii.edu for appointment and/or more information.