The Hilo Core Genomics Facility provides DNA sequencing and DNA Fragment Separation and Detection services, available to UH faculty, visiting researchers, staff, students, as well as other federal, state and private institutions in the area. Additional services are available for a fee. See our price list for available services and pricing.
The Core Facility offers DNA sequencing services, which can be used to determine nucleotide sequences of DNA samples such as PCR products and plasmids. Samples are prepared by the users (see Sample Preparation Sheet (Word)) and are submitted to the Core Facility, along with a Sample Sheet Submission (Excel).The Facility in turn processes the submitted samples by performing asequencing reaction with the Big Dye Terminator v3.1 reagents. This isfollowed by post-sequencing reaction clean-up using BigDye XTerminatorPurification Kit, and subsequent sample-loading into the AppliedBiosystems (AB) 3500 Genetic Analyzer instrument. The raw data iscollected and analyzed by the AB internal software, and the resultingsequence data is then made available to the user.
To sequence small genomes or a number of genes in multiple individuals, the Core Genomics Facility will be offering sequencing services using an Ion Torrent Personal Genome Machine (PGM). This technology provides high through-put sequencing using a chip. For more information on pricing and specific services, contact the Lab Manager.
DNA Fragment Separation and Detection Services
The Core Facility offers DNA Fragment and Detection services for customer-prepared samples. Samples are prepared by the users and are submitted to the Core Facility, along with a Sample Sheet (Word). DNA fragments are separated using the Applied Biosystems 3500 Genetic Analyzer or the Beckman Coulter Ceq 8000 Genetic Analysis System's capillary electrophoresis and are then made available to the user.
- Sample Preparation (call for more information)
- DNA Fragment Separation & Detection Services Sample Sheet (Word)
Orchid Virus Detection
Services to detect the common orchid RNA viruses Cymbidium mosaic potexvirus (CymMV) and Odontoglossum ringspot tobamovirus (ORSV) are available at the Core Facility. This is especially useful for farmers who need to know if their orchids are contaminated so that they can safely reproduce and sell them. Once an orchid tissue sample from a leaf or a flower is provided, the lab can provide results of the test rapidly. For more information, contact the Lab Manager.
Services to determine the sex of a variety of bird species are offered by the Core Facility. Once a blood sample is provided by the client, results of the test will be available quickly. For more information on pricing and specific services, contact the Lab Manager. See a sample bird sexing submission sheet (Word).
The Core Genomics Facility can also provide other itemized services such as DNA extraction, or service packages such as DNA extraction to sequenced product. Please contact the Lab Manager with your request or for more information, and see our current price list below.
Please see the current price list for services available at the Hilo Core Genomics Facility.
- Billing Information (Word)
- This document explains the billing procedures of the Core Facility, including how to remit payment for services rendered.